Document Details

Document Type : Thesis 
Document Title :
THE INDUCTION OF APOPTOSIS BY KOLA NUT EXTRACT AS RECENT AND PROMISING TREATMENT FOR LEUKEMIA
استحثاث موت الخلايا المبرمج باستخدام مستخلص نبتة الكولا (القورو) وامكانية استخدامها كعلاج لسرطان الدم
 
Subject : Faculty of Applied Medical Sciences 
Document Language : Arabic 
Abstract : Among several types of malignancies, leukemia is one of the most aggressive types of cancer with poor prognosis. Although traditional leukemia treatments are effective, several side effects could be developed. Alternative therapy options such as medicinal herbs that possess anticancer activity were investigated. Recently, the potential uses of Kola nut extracts have been considered a promised novel treatment for several forms of cancer including breast and prostate cancers. Because the efficiency of this natural plant has not been previously identified on leukemia before, based on that, we investigated the effect of this attractive potential anticancer medicinal plant in a human myelogenous leukemia cell line K562 at different concentration levels from 50 to 250µg/ml to evaluate its ability to treat leukemia through induction of apoptosis, since cancer cells are usually unable to drive this mechanism. This potential targeted action was investigated in K562 cells; by Resazurin and Trypan-blue dye exclusion assays for cell viability, and flow-cytometric analysis of Annexin V-FITC/Propidium Iodide, and 7-AAD stained cells for evaluation of apoptotic cells and progression of cell cycle, respectively. Results confirm that K562 cells were clearly affected by Kola nut extract, where the extract obviously inhibited the proliferation of leukemia cells and it successfully induced cell death, early and late apoptosis, by approximately 30% after 24 and 48 hours incubation. Interestingly, it is found that the inhibitory effect of Kola nut extract on K562 cells was gradually increased in a dose-and time-dependent manner. In addition, it was noted that Kola nut extract prompted cell cycle arrest in G2/M phase in a dose and time dependent fashion. In conclusion, these initial results suggest that Kola nut extract may successfully be great therapeutic possibility agent obtained from natural plants against myelogenous leukemia. They provide new insights in understanding the mechanisms of Kola nut extract. (298 words) 
Supervisor : Dr.Talal Gadah 
Thesis Type : Master Thesis 
Publishing Year : 1439 AH
2018 AD 
Added Date : Thursday, March 29, 2018 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
حمده عنيزان السعيديAL-Saeedi, Hamdah EenizanResearcherMaster 

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